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Int J Mol Med. 2018 Apr;41(4):2350-2358. doi: 10.3892/ijmm.2018.3426. Epub 2018 Jan 25.

miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway.

Author information

1
Department of Vascular Surgery, General Hospital of People's Liberation Army, Beijing 100853, P.R. China.
2
Department of Cardiology, Xi'an No. 3 Hospital, Xi'an, Shaanxi 710018, P.R. China.
3
Department of Cardiology, People's Hospital of Yuncheng, Yuncheng, Shanxi 044000, P.R. China.
4
Department of Cardiology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710004, P.R. China.
5
Department of Nursing, Xi'an No. 3 Hospital, Xi'an, Shaanxi 710018, P.R. China.
6
Department of Cardiology, Xi Jing Hospital, Xi'an, Shaanxi 710032, P.R. China.

Abstract

In the present study, the function of microRNA (miR)‑195 on abdominal aortic aneurysm (AAA) and its possible mechanism were investigated. Reverse transcription‑quantitative polymerase chain reaction analysis was used to detect the expression of miR‑195 in patients with AAA. The expression levels of miR‑195 in patients with AAA were effectively increased. The present study also used miR‑195 mimics to increase the expression of miR‑195, and ELISA kits and western blot analysis were used to analyze the levels of interleukin (IL)‑1β and IL‑6, and the protein expression levels of matrix metalloproteinase (MMP)‑2, MMP‑9, tumor necrosis factor (TNF)‑α, nuclear factor (NF)‑κB, vascular endothelial growth factor (VEGF), phosphoinositide 3‑kinase (PI3K) and phosphorylated (p‑)Akt. The overexpression of miR‑195 promoted the levels of IL‑1β and IL‑6, induced the protein expression of MMP‑2 and MMP‑9, upregulated the protein expression of TNF‑α and NF‑κB, and suppressed the protein expression levels of VEGF, PI3K and p‑Akt in angiotensin II‑vascular smooth muscle cells. In addition, TNF‑α promoted the pre‑inflammatory effect of miR‑195 on the protein expression levels of TNF‑α and NF‑κB, levels of IL‑1β and IL‑6, and protein expression levels of MMP‑2 and MMP‑9 in the angiotensin II‑vascular smooth muscle cells. Suppression of PI3K promoted the pre‑inflammatory effect of miR‑195 on the protein expression of PI3K, p‑Akt and VEGF, the levels of IL‑1β and IL‑6, and the protein expression of MMP‑2 and MMP‑9 in angiotensin II‑vascular smooth muscle cells. Combined, these results suggested that miR‑195 suppressed AAA inflammation through the TNF‑α/NF‑κB and VEGF/PI3K/Akt pathways.

PMID:
29393364
DOI:
10.3892/ijmm.2018.3426
[Indexed for MEDLINE]

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