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Exp Dermatol. 2018 Jun;27(6):678-681. doi: 10.1111/exd.13505. Epub 2018 Apr 3.

An efficient method for eccrine gland isolation from human scalp.

Author information

1
Mediteknia Hair Transplant Clinic and Hair Lab, Las Palmas de Gran Canaria, Canary Islands, Spain.
2
Universidad Fernando Pessoa Canarias, Gran Canaria, Spain.
3
Medical Pathology Group, IUIBS, Universidad de Las Palmas Gran Canaria, Gran Canaria, Spain.
4
Monasterium Laboratory, Münster, Germany.
5
Reina Sofia University General Hospital, University of Murcia, Murcia, Spain.
6
The Centre for Dermatology Research, MAHSC, University of Manchester, Manchester, UK.
7
National Institutes of Health Biomedical Research Center, Manchester, UK.
8
Department of Dermatology, Miller School of Medicine, University of Miami, Miami, FL.

Abstract

We describe a simple and efficient method to isolate eccrine sweat glands from the human scalp. This method is inspired by the hair graft harvesting method used in hair transplantation. Based on the recently described anatomical relationship between the scalp hair follicle and the eccrine gland, we have found that scalp follicular unit grafts are an excellent eccrine gland isolation source, especially for the coiled component. In order to make the gland visible for stereoscopic microdissection, the follicular units need to be previously stained with a vital dye like methylene blue or neutral red. The simplicity and efficiency of this isolation method should encourage further research into human eccrine sweat gland function which has always been hindered by the difficulty of gland isolation.

KEYWORDS:

Eccrine glands; follicular unit; hair follicle; hair graft; hair transplantation; methylene blue; neutral red; sweat; sweat glands

PMID:
29392864
DOI:
10.1111/exd.13505

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