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Methods Mol Biol. 2018;1741:111-123. doi: 10.1007/978-1-4939-7659-1_9.

Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro.

Author information

1
Microvascular and Molecular Neuro-Oncology Laboratory, Department of Pathology, NYU Langone Medical Center, New York, NY, USA.
2
Department of Pathology, NYU Langone Medical Center, New York, NY, USA.
3
Department of Pathology and Laboratory Medicine, Division of Neuropathology, Medical Center, University of California, Davis, Sacramento, CA, USA.
4
Microvascular and Molecular Neuro-Oncology Laboratory, Department of Pathology, NYU Langone Medical Center, New York, NY, USA. david.zagzag@nyumc.org.
5
Department of Pathology, NYU Langone Medical Center, New York, NY, USA. david.zagzag@nyumc.org.
6
Division of Neuropathology, Department of Neurosurgery, NYU Langone Medical Center, New York, NY, USA. david.zagzag@nyumc.org.
7
NYU Langone Laura and Isaac Perlmutter Cancer Center, New York, NY, USA. david.zagzag@nyumc.org.

Abstract

To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.

KEYWORDS:

Chemical pseudohypoxia; Glioblastoma; Glioma cell lines; Hypoxia cell culture chamber; Hypoxia-inducible factor-1α; Hypoxia-regulated genes; Immunocytochemistry; Prolyl hydroxylases; Tumor hypoxia; Western blot analysis

PMID:
29392695
DOI:
10.1007/978-1-4939-7659-1_9
[Indexed for MEDLINE]

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