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Methods Mol Biol. 2018;1741:53-62. doi: 10.1007/978-1-4939-7659-1_3.

Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens.

Author information

1
Department of Neurological Surgery, Washington University School of Medicine, St. Louis, MO, USA.
2
Department of Neurological Surgery, Washington University School of Medicine, St. Louis, MO, USA. alberthkim@wustl.edu.
3
Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA. alberthkim@wustl.edu.
4
Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA. alberthkim@wustl.edu.
5
Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, USA. alberthkim@wustl.edu.
6
Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MO, USA. alberthkim@wustl.edu.

Abstract

This chapter describes a method for isolation, maintenance, and propagation of primary glioblastoma (GBM) cells in adherent monolayer cultures from patient tumor specimens. This method enables the establishment of GBM cultures with stem or progenitor-like cell characteristics, including self-renewal capacity, differentiation along restricted neural lineages, and tumor-initiating potential when orthotopically injected into immunocompromised mice. This experimentally tractable model system is therefore suitable for a wide variety of analyses in vitro as well as in vivo. Key examples of biological analyses that can be performed using these cells are also described.

KEYWORDS:

Adherent culture; Glioblastoma; Glioblastoma stem-like cells; Monolayer culture; Tumor-initiating cells

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