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Sci Rep. 2018 Feb 1;8(1):2044. doi: 10.1038/s41598-018-20481-3.

Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke.

Kim YR1,2, Ahn SM2, Pak ME1,3, Lee HJ1,3, Jung DH1,3, Shin YI4, Shin HK1,2,3,5, Choi BT6,7,8,9.

Author information

1
Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea.
2
Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan, 50612, Republic of Korea.
3
Graduate Training Program of Korean Medicine for Healthy Aging, School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea.
4
Department of Rehabilitation Medicine, School of Medicine, Pusan National University, Yangsan, 50612, Republic of Korea.
5
Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea.
6
Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea. choibt@pusan.ac.kr.
7
Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan, 50612, Republic of Korea. choibt@pusan.ac.kr.
8
Graduate Training Program of Korean Medicine for Healthy Aging, School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea. choibt@pusan.ac.kr.
9
Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Yangsan, 50612, Republic of Korea. choibt@pusan.ac.kr.

Abstract

The beneficial effects of mesenchymal stem cells (MSCs) and electroacupuncture (EA) on neurogenesis and related trophic factors remain unclear. Bone marrow MSCs (mBMSC) were transplanted into the striatum of mice with middle cerebral artery occlusion (MCAO), and EA stimulation was applied at two acupoints, Baihui and Dazhui. EA treatment significantly improved motor function, and a synergistic effect of combined mBMSC and EA treatment was observed. Combined mBMSC and EA treatment reduced prominent atrophic changes in the striatum and led to proliferation of neural progenitor cells in the subventricular zone (SVZ) and the surrounding areas of the striatum (SVZ + striatum) of MCAO mice. The mBMSC and EA treatment markedly enhanced mature brain-derived neurotrophic factor (mBDNF) expression in the SVZ + striatum and hippocampus of mice with MCAO, and combined treatment enhanced neurotrophin-4 (NT4) expression. The number of mBDNF- and NT4-positive neurons in the SVZ + striatum and hippocampus increased following EA treatment. Combined treatment led to an increase in the expression levels of phosphorylated cAMP response element binding protein in the neuroblasts of the striatum. Our results indicate that combined MSC and EA treatment may lead to a better therapeutic effect via co-regulation of neurotrophic factors in the brain, by regulating neurogenesis more than single therapy.

PMID:
29391466
PMCID:
PMC5794924
DOI:
10.1038/s41598-018-20481-3
[Indexed for MEDLINE]
Free PMC Article

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