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Eur J Immunol. 1986 May;16(5):492-7.

Influence of carriers on the development and localization of anti-2,4,6-trinitrophenyl (TNP) antibody-forming cells in the murine spleen. II. Suppressed antibody response to TNP-Ficoll after elimination of marginal zone cells.


The macrophages in the marginal zones in mice spleens were eliminated by i.v. injection of dichloromethylene diphosphonate encapsulated in liposomes. After immunization with a thymus-dependent (trinitrophenylated keyhole limpet hemocyanin; TNP-KLH) or thymus-independent type 1 (TNP-lipopolysaccharide) antigen, no differences in antibody responses in treated and untreated mice were observed. However, immunization with a thymus-independent type 2 (TI-2) antigen (TNP-Ficoll) resulted in a strong decrease of the antibody response in macrophage-depleted animals. Anti-TNP serum levels dropped 10-fold and the number of antibody-forming cells in the spleen 30-60-fold. These results confirm the special role of the marginal zone macrophages in the processing of TI-2 antigens, as earlier suggested in splenectomy studies.

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