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Immun Inflamm Dis. 2018 Jun;6(2):245-255. doi: 10.1002/iid3.213. Epub 2018 Feb 1.

Collagen-derived peptides modulate CD4+ T-cell differentiation and suppress allergic responses in mice.

Author information

1
Department of Biosciences, School of Science, Kitasato University, Sagamihara, Kanagawa, Japan.
2
Research Institute of Biomatrix, Nippi Inc., Toride, Ibaraki, Japan.
3
Institute for Animal Reproduction, Kasumigaura, Ibaraki, Japan.

Abstract

INTRODUCTION:

Collagen peptides have been widely used as a food supplement. After ingestion of collagen peptides, oligopeptides containing hydroxyproline (Hyp), which are known to have some physiological activities, are detected in peripheral blood. However, the effects of collagen-peptide administration on immune response are unclear. In the present study, we tested the effects of collagen-peptide ingestion on allergic response and the effects of collagen-derived oligopeptides on CD4+ T-cell differentiation.

METHODS:

BALB/c mice fed a collagen-peptide diet were immunized with ovalbumin (OVA), and their serum IgE and IgG levels, active cutaneous anaphylaxis, and cytokine secretion by splenocytes were examined. Naive CD4+ T cells were stimulated with anti-CD3 and anti-CD28 in the presence of collagen-derived oligopeptides, and the expression of IFN-γ, IL-4, and Foxp3 was analyzed.

RESULTS:

In an active anaphylaxis model, oral administration of collagen peptides suppressed serum OVA-specific immunoglobulin E (IgE) production and diminished anaphylaxis responses. In this model, the ingestion of collagen peptides skewed the pattern of cytokine production by splenocytes toward T-helper (Th) type 1 and regulatory T (Treg) cells. In vitro T-helper cell differentiation assays showed that Hyp-containing oligopeptides promoted Th1 differentiation by upregulating IFN-γ-induced signal transducer and activator of transcription 1 (STAT1) signaling. These oligopeptides also promoted the development of Foxp3+ Treg cells in response to antigen stimulation in the presence of TGF-β.

CONCLUSIONS:

Collagen-peptide ingestion suppresses allergic responses by skewing the balance of CD4+ T cells toward Th1 and Treg cells and seems to be a promising agent for preventing allergies and inflammatory diseases.

KEYWORDS:

Active cutaneous anaphylaxis model; T cells; collagen peptide

PMID:
29388365
PMCID:
PMC5946155
DOI:
10.1002/iid3.213
[Indexed for MEDLINE]
Free PMC Article

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