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Methods Mol Biol. 2018;1740:43-57. doi: 10.1007/978-1-4939-7652-2_5.

Isolation of Extracellular RNA from Serum/Plasma.

Author information

1
Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.
2
Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
3
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
4
Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
5
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
6
Program in Neuroscience, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
7
Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA. llaurent@ucsd.edu.
8
Center for RNA Interference and Non-Coding RNAs, MDACC, Houston, TX, USA.
9
Department of Cancer Biology, MDACC, Houston, TX, USA.

Abstract

Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

KEYWORDS:

Cell biology; Extracellular RNA; Extracellular Vesicle; Isolation; Molecular Biology; Nucleic acids; Purification and separation

PMID:
29388135
DOI:
10.1007/978-1-4939-7652-2_5
[Indexed for MEDLINE]

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