Format

Send to

Choose Destination
Methods Mol Biol. 2018;1740:35-41. doi: 10.1007/978-1-4939-7652-2_4.

Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture.

Author information

1
Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA.
2
Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA. Patel.Tushar@mayo.edu.

Abstract

Current approaches for collection of extracellular vesicles (EV) are based on classical cell culture media production. This involves collection from cells grown in flasks, and can require multiple rounds of centrifugation or filtration, followed by ultracentrifugation or density gradient centrifugation. There are several limitations of these approaches, for example, they require a large input volume, the yield and concentration is low, and the process is time consuming. Most cell cultures require the use of fetal bovine serum which contains a large amount of endogenous EV that can contaminate isolations of cell-derived EVs. The use of cell cultures within a hollow fiber bioreactor could address many of these limitations and produce a continuous source of highly concentrated EVs without contamination from serum EVs, and that are suitable for downstream applications.

KEYWORDS:

Exosomes; Extracellular vesicles; Hollow fiber bioreactor; Isolation

PMID:
29388134
DOI:
10.1007/978-1-4939-7652-2_4
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center