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Acta Biochim Biophys Sin (Shanghai). 2018 Mar 1;50(3):238-245. doi: 10.1093/abbs/gmy004.

C-reactive protein is associated with the development of tongue squamous cell carcinoma.

Author information

1
School and Hospital of Stomatology, Shandong University, Jinan 250012, China.
2
Department of Stomatology, Zibo Central Hospital affiliated to Shandong University, Zibo 255000, China.
3
Department of Breast and Thyroid Surgery, Zibo Central Hospital affiliated to Shandong University, Zibo 255000, China.
4
Department of Stomatology, Qilu Hospital of Shandong University, Jinan 250012, China.

Abstract

C-reactive protein (CRP) acts as a biomarker reflecting different degrees of inflammation. Accumulating reports have suggested that there is a close relationship between CRP and various cancers. However, the influence of CRP on the development of tongue squamous cell carcinoma (TSCC) remains unclear. The purpose of this study was to investigate the role of CRP in TSCC. The results of immunohistochemical staining and statistical analyses showed that CRP expression was associated with TSCC tumor size, lymph node metastasis and pathological differentiation. Cell Counting Kit-8 (CCK-8) assay revealed that CRP could enhance TSCC cell proliferation in a dose- and time-dependent manner. Moreover, with CRP stimulation, proliferating cell nuclear antigen (PCNA) expression patterns presented a notable time-dependent up-regulation. In addition, CRP could enhance the invasion and migration of TSCC cells, as revealed by transwell and wound-healing assays, respectively. Annexin V-FITC/PI staining showed that CRP could protect TSCC cells from starvation- and drug-induced apoptosis. With CRP stimulation, the protein expression levels of phosphorylated protein kinase B (pAkt), phosphorylated mammalian target of rapamycin (pmTOR) and phosphorylated S6 ribosomal protein (pS6) were significantly increased, as demonstrated by western blot analysis. Our data suggest that CRP may play an important role in the development of TSCC. Moreover, the biological effects of CRP on TSCC cells might be related to Akt, mTOR, and S6.

PMID:
29385406
DOI:
10.1093/abbs/gmy004
[Indexed for MEDLINE]

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