Format

Send to

Choose Destination
Clin Exp Reprod Med. 2017 Dec;44(4):201-206. doi: 10.5653/cerm.2017.44.4.201. Epub 2017 Dec 31.

Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation.

Kim SW1,2, Jee BC2,3, Kim SK2,3, Kim SH1,2.

Author information

1
Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
2
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
3
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract

Objective:

The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure.

Methods:

Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined.

Results:

The percentage of sperm cells with fragmented DNA was significantly lower in the swim-up fraction (9.7%, p=0.001) than in the unprocessed fraction (27.0%), but not in the DGC fraction (27.8%, p=0.098). The percentage of sperm cells with normal X or Y chromosomes was comparable in the three fractions. The percentage of sperm cells with abnormal chromatin structure significantly decreased after DGC (from 15.7% to 10.3%, p=0.002). The swim-up method also tended to reduce the percentage of sperm cells with abnormal chromatin structure, but the difference was not significant (from 15.7% to 11.6%, p=0.316).

Conclusion:

The swim-up method is superior for enriching genetically competent sperm.

KEYWORDS:

Aneuploidy; DNA fragmentation; Density gradient centrifugation; Sperm; Swim-up

Conflict of interest statement

Conflict of interest: No potential conflict of interest relevant to this article was reported.

Supplemental Content

Full text links

Icon for The Korean Society for Reproductive Medicine Icon for PubMed Central
Loading ...
Support Center