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Toxicol Res. 2018 Jan;34(1):31-39. doi: 10.5487/TR.2018.34.1.031. Epub 2018 Jan 15.

Antioxidant and Skin Anti-Aging Effects of Marigold Methanol Extract.

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Department of Physical Therapy, Daejeon Woori Hospital, Daejeon, Korea.
Department of Beauty Care Design, Halla University, Wonju, Korea.
Department of Public Health, Graduate School, Keimyung University, Daegu, Korea.


The objective of this study was to evaluate the antioxidant and anti-aging effects of marigold methanol extract (MGME) in human dermal fibroblasts. Total polyphenolic and flavonoid contents in MGME were 74.8 mg TAE (tannic acid equivalent)/g and 85.6 mg RE (rutin equivalent)/g, respectively. MGME (500 μg/mL) increased 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging, and superoxide dismutase (SOD)-like antioxidant activities by 36.5, 54.7, and 14.8%, respectively, compared with the control. At 1,000 μg/mL, these activities increased by 63.7, 70.6, and 20.6%, respectively. MGME (100 μg/mL) significantly increased the synthesis of type 1 procollagen by 83.7% compared with control treatment. It also significantly decreased Matrix Metalloproteinase-2 (MMP-2) activity and MMP-1 mRNA expression by 36.5% and 69.5%, respectively; however, it significantly increased laminin-5 mRNA expression by 181.2%. These findings suggest that MGME could protect human skin against photo-aging by attenuating oxidative damage, suppressing MMP expression and/or activity as well as by stimulating collagen synthesis.


Antioxidant activity; MMP-1 mRNA; MMP-2 activity; Marigold methanol extract; Skin anti-aging effects; Type I procollagen synthesis

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