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Toxicol Res. 2018 Jan;34(1):13-21. doi: 10.5487/TR.2018.34.1.013. Epub 2018 Jan 15.

Inhibition of Adipocyte Differentiation by Anthocyanins Isolated from the Fruit of Vitis coignetiae Pulliat is Associated with the Activation of AMPK Signaling Pathway.

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Department of Applied Research, National Marine Biodiversity Institute of Korea, Seocheon, Korea.
Open Laboratory for Muscular and Skeletal Disease, and Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, Korea.
Anti-Aging Research Center, Dongeui University, Busan, Korea.
Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan, Korea.
Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dongeui University, Busan, Korea.


Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.


3T3-L1 preadipocytes; AMPK; Adipogenesis; Anthocyanins; Vitis coignetiae pulliat

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