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Toxicol Res. 2018 Jan;34(1):13-21. doi: 10.5487/TR.2018.34.1.013. Epub 2018 Jan 15.

Inhibition of Adipocyte Differentiation by Anthocyanins Isolated from the Fruit of Vitis coignetiae Pulliat is Associated with the Activation of AMPK Signaling Pathway.

Author information

1
Department of Applied Research, National Marine Biodiversity Institute of Korea, Seocheon, Korea.
2
Open Laboratory for Muscular and Skeletal Disease, and Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, Korea.
3
Anti-Aging Research Center, Dongeui University, Busan, Korea.
4
Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan, Korea.
5
Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dongeui University, Busan, Korea.

Abstract

Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.

KEYWORDS:

3T3-L1 preadipocytes; AMPK; Adipogenesis; Anthocyanins; Vitis coignetiae pulliat

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