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J Clin Biochem Nutr. 2018 Jan;62(1):63-67. doi: 10.3164/jcbn.16-103. Epub 2017 Oct 11.

The expression of Toll-like receptor 5 in preterm histologic chorioamnionitis.

Author information

1
Department of Obstetrics and Gynecology, Nagoya Graduate University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
2
Department of Obstetrics and Gynecology, Handa City Hospital, 2-29 Toyo-cho, Handa-shi, Aichi 475-8559, Japan.
3
Laboratory of Bell Research Centre-Department of Obstetrics and Gynaecology Collaborative Research, Nagoya Graduate University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
4
Department of Obstetrics and Gynecology, Japanese Red Cross Nagoya Daiichi Hospital, 3-35 Michishita-cho, Nakamura-ku, Nagoya 453-8511, Japan.
5
Department of Neurology, Yanbian University Hospital, 1327 JuZi Street, Yanji City, JiLin Province 133000, China.
6
Office of international Affairs, Nagoya Graduate University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
7
Department of Healthcare Administration, Nagoya Graduate University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

Abstract

Spontaneous preterm birth is often caused by chorioamnionitis. Toll-like receptors (TLRs) have a role in the response of the innate immune system. The role of TLR5 in chorioamnionitis remains unclear: however, TLR5 was reported to have a significantly stronger effect on the induction of interleukin (IL)-6 when compared with other TLRs in amniotic epithelial cells. The aim of this study was to investigate TLR5 expression in placentas with preterm histologic chorioamnionitis (HCA). The expression levels of TLR5 were evaluated in the amnions, chorions, deciduae and villi with and without HCA using immunohistochemistry. The co-localization of IL-6 or IL-8 with TLR5 was examined by immunofluorescence. The production of IL-6 was examined in primary tissue cultured fetal membranes treated with and without the TLR5 agonist. The protein expression of TLR5 was significantly increased in amnions with HCA (p<0.05) and showed a trend toward an increase in chorions with HCA, whereas no significant difference was detected in the villi and decidua. TLR5 co-localized with IL-6 and IL-8 in amnions and chorions. IL-6 showed a significant increase (p<0.05) with the TLR5 agonist. These results suggest that TLR5 plays a role in the pathogenesis of preterm HCA and IL-6 production.

KEYWORDS:

IL-6; IL-8; amnion; chorion

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