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Phytother Res. 2018 May;32(5):933-942. doi: 10.1002/ptr.6035. Epub 2018 Jan 24.

Superior anticancer activity is demonstrated by total extract of Curcuma longa L. as opposed to individual curcuminoids separated by centrifugal partition chromatography.

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Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodźki, 20-093, Lublin, Poland.
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 1 Chodźki, 20-093, Lublin, Poland.
Department of Pathophysiology, Medical University of Lublin, 8 Jaczewskiego, 20-090, Lublin, Poland.
Department of Food and Nutrition, Medical University of Lublin, 4a Chodźki., 20-093, Lublin, Poland.
Armen Instrument Application Laboratory, ZI Kermelin, 56890, Saint Avé, France.
Immunocon Biotech Sp. z o. o., 39A Zana, 20-601, Lublin, Poland.
Department of Cosmetology, University of Information Technology and Management, 2 Sucharskiego, 35-225, Rzeszów, Poland.


Three curcuminoids: bisdemethoxycurcumin, demethoxycurcumin, and curcumin from turmeric were successfully separated by a high capacity solvent system composed of heptane: chloroform: methanol: water mixture (5: 6: 3: 2 v/v/v/v) tailored for centrifugal partition chromatographs at K-values of 0.504, 1.057, 1.644, respectively. These three ferulic acid derivatives obtained at a purity rate exceeding 95% were analysed by an HPLC-MS spectrometer. Turmeric extract inhibited the proliferation/viability of A549 human lung cancer, HT29 colon cancer, and T98G glioblastoma cell lines in (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay (MTT). Single curcuminoids significantly decreased the viability/proliferation of lung cancer cells in a dose-dependent manner. However, total extract displayed the superior anticancer activity in the investigated cell lines. Crude extract in combination with cisplatin augmented the decrease in the viability of cancer cells compared with single compound treatment in A549 lung cancer cells. Total extract of Curcuma longa could be regarded as being more effective against lung cancer cells in vitro than its separated compounds.


CPC; Curcuma longa; LC-Q-TOF-MS; Zingiberaceae; cisplatin; small cell lung cancer

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