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World J Pediatr. 2018 Jun;14(3):233-237. doi: 10.1007/s12519-017-0102-x. Epub 2018 Jan 24.

Serum proteomic profiling for autism using magnetic bead-assisted matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a pilot study.

Author information

1
Department of Health Care, Xi'an Children's Hospital, Xi'an Jiaotong University, 69 Xijuyuanxiang, Xi'an, 710002, China. chenyannichil@163.com.
2
Department of Health Care, Xi'an Children's Hospital, Xi'an Jiaotong University, 69 Xijuyuanxiang, Xi'an, 710002, China.
3
Department of Biology, College of Liberal Arts and Science, The University of Iowa, Iowa, USA.
4
Xi'an Maternal and Child Health Hospital, 73 West Street, Xi'an, 710002, China.
5
Department of Pediatrics, Shaanxi University of Chinese Medicine, Xianyang, Shannxi, China.

Abstract

BACKGROUND:

The pathogenesis of autism spectrum disorders remains elusive and currently there are no diagnostic or predictive biomarkers in autism available. Proteomic profiling has been used in a wide range of neurodevelopmental disorder studies, which could produce deeper perceptions of the molecular bases behind certain disease and potentially becomes useful in discovering biomarkers in autism spectrum disorders.

METHODS:

Serum samples were collected from autistic children about 3 years old in age (n = 32) and healthy controls (n = 20) in similar age and gender. The samples were identified specific proteins that are differentially expressed by magnetic bead-based pre-fractionation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS).

RESULTS:

Eight protein peaks were significantly different in autistic children from the healthy controls (P < 0.0001). The two peaks with the most significant differences were 6428 and 7758 Da in size.

CONCLUSION:

According to differences in serum protein profiles between the autistic children and healthy controls, this study identified a set of differentially expressed proteins those are significant for further evaluation and might function as biomarkers in autism.

KEYWORDS:

Autism; Matrix-assisted laser desorption ionization time-of-flight mass spectrometry; Proteomic profiling; Weak cation-exchange chromatography magnetic beads

PMID:
29368242
DOI:
10.1007/s12519-017-0102-x
[Indexed for MEDLINE]

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