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Mol Cell Biochem. 2018 Aug;445(1-2):169-178. doi: 10.1007/s11010-017-3262-z. Epub 2018 Jan 24.

Falcarindiol inhibits LPS-induced inflammation via attenuating MAPK and JAK-STAT signaling pathways in murine macrophage RAW 264.7 cells.

Author information

1
Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University, 861-1 Chongnung-dong, Songbuk-gu, Seoul, 136-702, South Korea.
2
Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University, 861-1 Chongnung-dong, Songbuk-gu, Seoul, 136-702, South Korea. ykkim@kookmin.ac.kr.

Abstract

Falcarindiol (FAD) is a natural polyacetylene compound found rich in many plants of the Umbelliferae family. Previously, we isolated FAD from the rhizome of Cnidium officinale Makino, which belongs to the Umbelliferae family and found it to have a significant inhibitory effect on lipopolysaccharide (LPS)-induced production of nitric oxide, a pro-inflammatory molecule in murine macrophage RAW 264.7 cells. In this study, we investigated its effect on the expression of other major pro-inflammatory molecules as well as the mechanism underlying these effects. Pre-treatment of RAW 264.7 cells with FAD suppressed LPS-stimulated mRNA expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) and thereby reduced the respective protein levels. Mechanistic studies demonstrated that FAD attenuated the LPS-induced activation of JNK, ERK, STAT1, and STAT3 signaling molecules. Moreover, we found that FAD did not influence LPS-induced activation of p38 and NFκB signaling pathways. Collectively, this study provides evidence that FAD inhibits the production of major pro-inflammatory molecules in LPS-challenged murine macrophages via suppression of JNK, ERK, and STAT signaling pathways.

KEYWORDS:

Anti-inflammatory activity; Cnidium officinale; Falcarindiol; RAW 264.7 cells; Rhizome

PMID:
29368095
DOI:
10.1007/s11010-017-3262-z
[Indexed for MEDLINE]

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