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Diagn Microbiol Infect Dis. 2018 May;91(1):6-12. doi: 10.1016/j.diagmicrobio.2017.12.017. Epub 2017 Dec 24.

An advanced uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) technique used in the sensitive and specific detection of Cryptosporidium parvum, Cryptosporidium hominis, and Cryptosporidium meleagridis in AIDS patients.

Author information

1
Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Medical Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Electronic address: Falahi.sh@lums.ac.ir.
2
Department of Medical Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
3
Department of Communicable Disease Control and Prevention, Deputy of Health, Lorestan University of Medical Sciences, Khorramabad, Iran.
4
Medical Student, Faculty of Medicine, University of Debrecen, Hungary.
5
Department of Biotechnology, Islamic Azad University, Damghan Branch, Damghan, Iran.
6
Department of Immunology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
7
Department of Epidemiology and Statistics, Behavioral Sciences Research Center, Isfahan University of Medical Science, Isfahan, Iran.
8
School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

Abstract

The rapid and accurate detection of Cryptosporidium spp. is critically important for the prevention and timely treatment of cryptosporidiosis in AIDS patients (APs). This study was conducted to examine a UDG-LAMP technique for the first time to diagnose cryptosporidiosis in APs. After collecting demographic and clinical data, three stool samples were collected from the participants (120 volunteering APs). The microscopic examination of stained smears using the acid-fast method and the UDG-LAMP assay were performed for each sample. 10% of APs were infected with Cryptosporidium spp. The number of detected cryptosporidiosis cases using the acid-fast staining and UDG-LAMP methods were significantly different (P < 0.001). Diarrhea and weight loss were found to be significantly associated with cryptosporidiosis in patients (P < 0.05). The pretreatment of LAMP reagents with UDG successfully eliminated the likelihood of product re-amplification remaining from previous reactions. The UDG-LAMP technique could detect cryptosporidiosis in APs with high sensitivity and rapidity without carryover contamination.

KEYWORDS:

AIDS patients; Cryptosporidium spp.; Detection; Rapid; Sensitive; UDG-LAMP

[Indexed for MEDLINE]

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