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J Infect Dis. 2018 Mar 28;217(8):1247-1256. doi: 10.1093/infdis/jiy026.

Comparison of Palivizumab-Like Antibody Binding to Different Conformations of the RSV F Protein in RSV-Infected Adult Hematopoietic Cell Transplant Recipients.

Author information

1
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX.
2
Department of Epidemiology and Biostatistics, University of Texas Health Science Center at San Antonio, University of Texas, Houston.
3
Departments of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center, University of Texas, Houston.
4
Department of Pediatrics, Baylor College of Medicine, Houston, TX.

Abstract

Background:

Most respiratory syncytial virus (RSV) vaccine candidates include fusion (F) protein in different conformations. Antigenic site II found in the different F conformations is the target of palivizumab, the only US Food and Drug Administration approved monoclonal antibody (mAb). Serum palivizumab-like antibody (PLA) is a potential serologic correlate of immunity. Our objective was to determine if different conformations of F protein in a palivizumab competitive antibody (PCA) assay affect the PLA concentrations.

Methods:

Four PCA assays were standardized using mAbs. Each contained prefusion, postfusion, or intermediate F forms. PLA concentrations were measured in acute and convalescent sera from 22 RSV/A and 18 RSV/B-infected adult hematopoietic cell transplant (HCT) recipients. PLA concentrations were calculated using a 4-parameter logistic regression model and analyzed for statistical significance.

Results:

PCA assays revealed significantly greater PLA concentrations in convalescent sera; comparable increases in PLA concentration in RSV/A and RSV/B-infected HCT recipients; and significantly reduced PLA concentrations in HCT recipients who shed RSV ≥14 days. A significant positive correlation was observed between PCA assays and RSV neutralizing antibody titers.

Conclusions:

F protein conformation does not appear to have a measurable impact on PCA assays for measuring PLA induced by RSV/A or RSV/B infection.

PMID:
29365155
DOI:
10.1093/infdis/jiy026
[Indexed for MEDLINE]

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