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Int J Mol Sci. 2018 Jan 24;19(2). pii: E350. doi: 10.3390/ijms19020350.

Comparative Digital Gene Expression Analysis of Tissue-Cultured Plantlets of Highly Resistant and Susceptible Banana Cultivarsin Response to Fusarium oxysporum.

Author information

1
College of Horticulture, South China Agricultural University, Guangzhou 510642, China. 13424453254@163.com.
2
College of Horticulture, South China Agricultural University, Guangzhou 510642, China. mococo0619@yeah.net.
3
Institute of Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China. suzuxiang@163.com.
4
College of Horticulture, South China Agricultural University, Guangzhou 510642, China. hbchen@scau.edu.cn.
5
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, 783 01 Olomouc, Czech Republic. tomas.takac@upol.cz.
6
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, 783 01 Olomouc, Czech Republic. jozef.samaj@upol.cz.
7
College of Horticulture, South China Agricultural University, Guangzhou 510642, China. chxxu@scau.edu.cn.

Abstract

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the most destructive soil-borne diseases. In this study, young tissue-cultured plantlets of banana (Musa spp. AAA) cultivars differing in Foc susceptibility were used to reveal their differential responses to this pathogen using digital gene expression (DGE). Data were evaluated by various bioinformatic tools (Venn diagrams, gene ontology (GO) annotation and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses) and immunofluorescence labelling method to support the identification of gene candidates determining the resistance of banana against Foc. Interestingly, we have identified MaWRKY50 as an important gene involved in both constitutive and induced resistance. We also identified new genes involved in the resistance of banana to Foc, including several other transcription factors (TFs), pathogenesis-related (PR) genes and some genes related to the plant cell wall biosynthesis or degradation (e.g., pectinesterases, β-glucosidases, xyloglucan endotransglucosylase/hydrolase and endoglucanase). The resistant banana cultivar shows activation of PR-3 and PR-4 genes as well as formation of different constitutive cell barriers to restrict spreading of the pathogen. These data suggest new mechanisms of banana resistance to Foc.

KEYWORDS:

DGE; GO annotation; KEGG pathways; Venn diagram; banana Fusarium wilt; resistance genes

PMID:
29364855
PMCID:
PMC5855572
DOI:
10.3390/ijms19020350
[Indexed for MEDLINE]
Free PMC Article

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