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J Biol Chem. 2018 Mar 23;293(12):4403-4410. doi: 10.1074/jbc.M117.817890. Epub 2018 Jan 23.

The arrestin-1 finger loop interacts with two distinct conformations of active rhodopsin.

Author information

1
From the Institut für Medizinische Physik und Biophysik (CC2), Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany, melgeti@ucla.edu.
2
From the Institut für Medizinische Physik und Biophysik (CC2), Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
3
Group ProteInformatics.
4
Group Protein X-ray Crystallography and Signal Transduction.
5
Institut für Medizinische Physik und Biophysik, Universität Leipzig, Härtelstrasse 16-18, 04107 Leipzig, Germany.
6
Institut für Biologie, Biophysikalische Chemie, Humboldt-Universität zu Berlin, Invalidenstrasse 42, 10115 Berlin, Germany.

Abstract

Signaling of the prototypical G protein-coupled receptor (GPCR) rhodopsin through its cognate G protein transducin (Gt) is quenched when arrestin binds to the activated receptor. Although the overall architecture of the rhodopsin/arrestin complex is known, many questions regarding its specificity remain unresolved. Here, using FTIR difference spectroscopy and a dual pH/peptide titration assay, we show that rhodopsin maintains certain flexibility upon binding the "finger loop" of visual arrestin (prepared as synthetic peptide ArrFL-1). We found that two distinct complexes can be stabilized depending on the protonation state of E3.49 in the conserved (D)ERY motif. Both complexes exhibit different interaction modes and affinities of ArrFL-1 binding. The plasticity of the receptor within the rhodopsin/ArrFL-1 complex stands in contrast to the complex with the C terminus of the Gt α-subunit (GαCT), which stabilizes only one specific substate out of the conformational ensemble. However, Gt α-subunit binding and both ArrFL-1-binding modes involve a direct interaction to conserved R3.50, as determined by site-directed mutagenesis. Our findings highlight the importance of receptor conformational flexibility and cytoplasmic proton uptake for modulation of rhodopsin signaling and thereby extend the picture provided by crystal structures of the rhodopsin/arrestin and rhodopsin/ArrFL-1 complexes. Furthermore, the two binding modes of ArrFL-1 identified here involve motifs of conserved amino acids, which indicates that our results may have elucidated a common modulation mechanism of class A GPCR-G protein/-arrestin signaling.

KEYWORDS:

Fourier transform IR (FTIR); G protein; G protein-coupled receptor (GPCR); arrestin; biased signaling; functional selectivity; rhodopsin

PMID:
29363577
PMCID:
PMC5868258
DOI:
10.1074/jbc.M117.817890
[Indexed for MEDLINE]
Free PMC Article

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