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Methods Mol Biol. 2018;1730:213-225. doi: 10.1007/978-1-4939-7592-1_14.

Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis.

Author information

1
Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
2
Department of Chemistry, University of Alberta, Edmonton, AB, Canada. liang.li@ualberta.ca.

Abstract

Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.

KEYWORDS:

Blood; Chemical isotope labeling; Dansylation; DmPA; LC-MS; Metabolomics

PMID:
29363075
DOI:
10.1007/978-1-4939-7592-1_14
[Indexed for MEDLINE]

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