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Methods Mol Biol. 2018;1730:103-110. doi: 10.1007/978-1-4939-7592-1_8.

LC-MS/MS Analysis of Bile Acids.

Author information

1
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
2
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany. gerhard.liebisch@ukr.de.

Abstract

Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.

KEYWORDS:

Bile acids; Electrospray ionization; Internal standards; Isomer separation; Liquid chromatography; Method; Quantification; Tandem mass spectrometry

PMID:
29363069
DOI:
10.1007/978-1-4939-7592-1_8
[Indexed for MEDLINE]

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