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Methods Mol Biol. 2018;1730:59-72. doi: 10.1007/978-1-4939-7592-1_4.

Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis.

Author information

1
Lipid Mediator Unit, Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University of London, London, UK. j.dalli@qmul.ac.uk.
2
Lipid Mediator Unit, Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University of London, London, UK.
3
Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Abstract

Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.

KEYWORDS:

Eicosanoids; Flux analysis; Lipid mediator metabololipidomics; Liquid chromatography-tandem mass spectrometry; Maresin; Profiling; Protectin; Resolvin

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