pH-Mediated molecular differentiation for fluorimetric quantification of chemotherapeutic drugs in human plasma

Chem Commun (Camb). 2018 Feb 6;54(12):1485-1488. doi: 10.1039/c7cc07668a.

Abstract

At present, drug dosage is based on standardised approaches that disregard pharmakokinetic differences between patients and lead to non-optimal efficacy and unnecessary side effects. In this work, we demonstrate the potential of pH-mediated fluorescence spectroscopy for therapeutic drug monitoring in complex media. We apply this principle to the simultaneous quantification of the chemotherapeutic prodrug Irinotecan and its active metabolite SN-38 from human plasma across the clinically relevant concentration range, i.e. from micromolar to nanomolar at molar ratios of up to 30 : 1.

MeSH terms

  • Antineoplastic Agents, Phytogenic / blood*
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / metabolism
  • Camptothecin / analogs & derivatives*
  • Camptothecin / blood
  • Camptothecin / chemistry
  • Camptothecin / metabolism
  • Fluorometry*
  • Humans
  • Hydrogen-Ion Concentration
  • Irinotecan
  • Prodrugs / chemistry
  • Prodrugs / metabolism*

Substances

  • Antineoplastic Agents, Phytogenic
  • Prodrugs
  • Irinotecan
  • Camptothecin