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J Cell Biol. 2018 Mar 5;217(3):1079-1095. doi: 10.1083/jcb.201610098. Epub 2018 Jan 22.

The adherens junction-associated LIM domain protein Smallish regulates epithelial morphogenesis.

Author information

1
Stem Cell Biology, Institute for Anatomy and Cell Biology, Georg-August-University Göttingen, Göttingen, Germany.
2
Developmental Genetics, Institute for Biology, University of Kassel, Kassel, Germany.
3
Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany.
4
Cluster of Excellence - Cellular Stress Response in Aging-Associated Diseases, Cologne, Germany.
5
Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
6
Max Planck Institute for Developmental Biology, Tübingen, Germany.
7
Institute for Genetics, University of Cologne, Cologne, Germany.
8
Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.
9
Institute for Genetics, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
10
Institute of Neurobiology, Cells-in-Motion Cluster of Excellence, University of Münster, Münster, Germany.
11
Institute for Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany.
12
Stem Cell Biology, Institute for Anatomy and Cell Biology, Georg-August-University Göttingen, Göttingen, Germany andreas.wodarz@uk-koeln.de.

Abstract

In epithelia, cells adhere to each other in a dynamic fashion, allowing the cells to change their shape and move along each other during morphogenesis. The regulation of adhesion occurs at the belt-shaped adherens junction, the zonula adherens (ZA). Formation of the ZA depends on components of the Par-atypical PKC (Par-aPKC) complex of polarity regulators. We have identified the Lin11, Isl-1, Mec-3 (LIM) protein Smallish (Smash), the orthologue of vertebrate LMO7, as a binding partner of Bazooka/Par-3 (Baz), a core component of the Par-aPKC complex. Smash also binds to Canoe/Afadin and the tyrosine kinase Src42A and localizes to the ZA in a planar polarized fashion. Animals lacking Smash show loss of planar cell polarity (PCP) in the embryonic epidermis and reduced cell bond tension, leading to severe defects during embryonic morphogenesis of epithelial tissues and organs. Overexpression of Smash causes apical constriction of epithelial cells. We propose that Smash is a key regulator of morphogenesis coordinating PCP and actomyosin contractility at the ZA.

PMID:
29358210
PMCID:
PMC5839775
DOI:
10.1083/jcb.201610098
[Indexed for MEDLINE]
Free PMC Article

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