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Cell Prolif. 2018 Apr;51(2):e12432. doi: 10.1111/cpr.12432. Epub 2018 Jan 22.

Extremely low-frequency electromagnetic fields accelerates wound healing modulating MMP-9 and inflammatory cytokines.

Author information

1
Department of Medicine and Aging Science, University "G. d'Annunzio", Chieti, Italy.
2
Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio", Chieti, Italy.
3
Department of Psychological, Humanistic and Territorial Sciences, University "G. d'Annunzio", Chieti, Italy.

Abstract

OBJECTIVES:

In our previous reports, we have demonstrated that extremely low-frequency electromagnetic fields (ELF-EMF) exposure enhances the proliferation of keratinocyte. The present study aimed to clarify effects of ELF-EMF on wound healing and molecular mechanisms involved, using a scratch in vitro model.

MATERIALS AND METHODS:

The wounded monolayer cultures of human immortalized keratinocytes (HaCaT), at different ELF-EMF and Sham exposure times were monitored under an inverted microscope. The production and expression of IL-1β, TNF-α, IL-18 and IL-18BP were measured by enzyme-linked immunosorbent assay and quantitative real-time PCR. The activity and the expression of matrix metalloproteinases (MMP)-2/9 was evaluated by zymography and Western blot analysis, respectively. Signal transduction proteins expression (Akt and ERK) was measured by Western blot.

RESULTS:

The results of wound healing in vitro assay revealed a significant reduction of cell-free area time-dependent in ELF-EMF-exposed cells compared to Sham condition. Gene expression and release of cytokines analysed were significantly increased in ELF-EMF-exposed cells. Our results further showed that ELF-EMF exposure induced the activity and expressions of MMP-9. Molecular data showed that effects of ELF-EMF might be mediated via Akt and ERK signal pathway, as demonstrated using their specific inhibitors.

CONCLUSIONS:

Our results highlight ability of ELF-EMF to modulate inflammation mediators and keratinocyte proliferation/migration, playing an important role in wound repair. The ELF-EMF accelerates wound healing modulating expression of the MMP-9 via Akt/ERK pathway.

PMID:
29357406
PMCID:
PMC6528910
DOI:
10.1111/cpr.12432
[Indexed for MEDLINE]
Free PMC Article

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