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Appl Microbiol Biotechnol. 2018 Mar;102(5):2351-2361. doi: 10.1007/s00253-018-8756-4. Epub 2018 Jan 22.

Enhancement of Schizochytrium DHA synthesis by plasma mutagenesis aided with malonic acid and zeocin screening.

Author information

1
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
2
College of Biological and Food Engineering, Huaihua University, Huaihua, China.
3
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China. 19891996@sina.com.
4
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China. bioprocessor@aliyun.com.

Abstract

Schizochytrium sp. accumulates valuable polyunsaturated fatty acid (PUFA), such as docosahexaenoic acid (DHA). In order to increase DHA synthesis in this microorganism, physical or chemical mutagenesis aided with powerful screening methods are still preferable, as its DHA synthetic pathway has not yet been clearly defined for gene manipulation. To breed this agglomerate microorganism of thick cell wall and rather large genome for increasing lipid content and DHA percentage, a novel strategy of atmospheric and room temperature plasma (ARTP) mutagenesis coupled with stepped malonic acid (MA) and zeocin resistance screening was developed. The final resulted mutant strain mz-17 was selected with 1.8-fold increased DHA production. Accompanied with supplementation of Fe2+ in shake flask cultivation, DHA production of 14.0 g/L on average was achieved. This work suggests that ARTP mutation combined with stepped MA and zeocin resistance screening is an efficient method of breeding Schizochytrium sp. of high DHA production, and might be applied on other microorganisms for obtaining higher desired PUFA products.

KEYWORDS:

Atmospheric and room temperature plasma (ARTP); Docosahexaenoic acid (DHA); Fe·EDTA; Malonic acid (MA); Schizochytrium sp.; Zeocin

PMID:
29356868
DOI:
10.1007/s00253-018-8756-4
[Indexed for MEDLINE]

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