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Cytometry A. 2018 Apr;93(4):402-405. doi: 10.1002/cyto.a.23331. Epub 2018 Jan 22.

OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment.

Author information

1
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109.

Abstract

This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.

KEYWORDS:

antigen-presenting cells; dendritic cells; high-dimensional cytometry; human PBMCs; human tissue; immunophenotyping; myeloid cells

PMID:
29356334
DOI:
10.1002/cyto.a.23331

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