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Spine J. 2018 Jun;18(6):1053-1061. doi: 10.1016/j.spinee.2018.01.007. Epub 2018 Jan 31.

Relationships between vitamin D and paraspinal muscle: human data and experimental rat model analysis.

Author information

1
Department of Neurosurgery, Kyungpook National University Hospital 130, Dongdeok-ro, Jung-gu, Daegu, 41944, Republic of Korea.
2
Department of Neurosurgery, Kyungpook National University Hospital 130, Dongdeok-ro, Jung-gu, Daegu, 41944, Republic of Korea. Electronic address: nskimkt7@gmail.com.
3
Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 16, Yatap-ro 65beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
4
Department of Rehabilitation Medicine, Dongsan Medical Center, School of Medicine, Keimyung University, 56, Dalseong-ro, Jung-gu, Daegu, 41931, Republic of Korea.
5
International Collaboration on Repair Discoveries (ICORD), University of British Columbia, Blusson Spinal Cord Centre, 818 West 10th Ave, Vancouver, BC, V5Z 1M9, Canada; Vancouver Spine Surgery Institute, Department of Orthopaedics, University of British Columbia, Blusson Spinal Cord Centre, 818 West 10th Ave, Vancouver, BC, V5Z 1M9, Canada.
6
Department of Neurosurgery, Seoul National University Hospital 101, Daehak-Ro, Jongno-Gu, Seoul, 110-744, Republic of Korea.

Abstract

BACKGROUND CONTEXT:

Vitamin D deficiency (VDD) has been closely linked with skeletal muscle atrophy in many studies, but to date no study has focused on the paraspinal muscle.

PURPOSE:

To verify paraspinal muscle changes according to serum vitamin D level.

STUDY DESIGN:

A cross-sectional study of patients who visited our hospital and an in vivo animal study.

METHODS:

We measured serum vitamin D concentration in 91 elderly women and stratified them according to their vitamin D status in three groups, control, vitamin D insufficiency, and VDD, and obtained magnetic resonance imaging data of the lumbar spine and evaluated the quality and quantity of the paraspinal muscles. Additionally, we designed experimental rat models for VDD and VDD replacement. Then, we analyzed the microcomputed tomography data and histologic data of paraspinal muscles, and the histologic data and reverse transcription-quantitative polymerase chain reaction data of intramyonuclear vitamin D receptor (VDR) in paraspinal muscle through comparison with control rats (n=25, each group). This work was supported by a Biomedical Research Institute grant ($40,000), Kyungpook National University Hospital (2014).

RESULTS:

In the human studies, a significant decrease was noted in the overall paraspinal muscularity (p<.05) and increase in fatty infiltration in the VDD group as compared with the other groups (p<.05). In the rat experiment, a decrease was noted in paraspinal muscle fiber size and VDR concentration and VDR gene expression level, and total muscle volume of the VDD rats as compared with the control rats (p<.05). Vitamin D replacement after VDD could partially restore the muscle volume, muscle fiber size, and intramyonuclear VDR concentration levels (p<.05) of the paraspinal muscles.

CONCLUSIONS:

VDD induces paraspinal muscle atrophy and decreases the intramyonuclear VDR concentration and VDR gene expression level in these muscles. Vitamin D replacement contributes to the recovery from atrophy and restoration of intramyonuclear VDR concentration in VDD status.

KEYWORDS:

Intramyonuclear vitamin D receptor; Paraspinal muscle; Spinal disease; Vitamin D; Vitamin D deficiency; Vitamin D replacement

PMID:
29355791
DOI:
10.1016/j.spinee.2018.01.007
[Indexed for MEDLINE]

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