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Urolithiasis. 2018 Nov;46(6):503-514. doi: 10.1007/s00240-018-1037-y. Epub 2018 Jan 20.

16S rRNA gene sequencing reveals altered composition of gut microbiota in individuals with kidney stones.

Tang R1,2,3, Jiang Y1,2,3, Tan A1,4, Ye J1,2,3, Xian X1,2,3, Xie Y1,2,3, Wang Q1,2,3, Yao Z5,6,7, Mo Z1,2,3,8.

Author information

1
Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, 530021, Guangxi, China.
2
Guangxi Key Laboratory of Genomic and Personalized Medicine, Nanning, Guangxi, China.
3
Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Nanning, Guangxi, China.
4
Department of Chemotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
5
Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, 530021, Guangxi, China. youziting@163.com.
6
Guangxi Key Laboratory of Genomic and Personalized Medicine, Nanning, Guangxi, China. youziting@163.com.
7
Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Nanning, Guangxi, China. youziting@163.com.
8
Institute of Urology and Nephrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.

Abstract

Nephrolithiasis is a common urological disease with high prevalence and recurrence rates. Characterizing gut microbiome profiles of nephrolithiasis patients may provide valuable insights and potential biomarkers for the disease. Therefore, we explored the relation between gut microbiome and nephrolithiasis using 16S ribosomal RNA (rRNA) gene sequencing. 13 patients with multiple kidney stones and 13 matched healthy controls were recruited. A decreasing trend in number of observed species in nephrolithiasis patients was detected, although statistical significance was not reached (pā€‰=ā€‰0.086). The inter-group variability in community structure by beta diversity analysis showed a clear separation between nephrolithiasis patients and healthy controls. Twenty genera differentiated significantly in relative abundance between nephrolithiasis patients and healthy controls (all pā€‰<ā€‰0.05). Among the 20 genera, Phascolarctobacterium, Parasutterella, Ruminiclostridium_5, Erysipelatoclostridium, Fusicatenibacter and Dorea were correlated with the concentration of the trace elements in blood, including potassium, sodium, calcium and chlorinum. Characteristic microbiome in nephrolithiasis patients was also identified by linear discriminant analysis effect size (LEfSe). These findings may provide novel and non-invasive potential diagnostic biomarkers for nephrolithiasis, and contribute to prevention and treatment of nephrolithiasis from the perspective of maintaining micro-ecological equilibrium in gut.

KEYWORDS:

16S rRNA; Biomarker; Gut microbiota; Nephrolithiasis

PMID:
29353409
DOI:
10.1007/s00240-018-1037-y
[Indexed for MEDLINE]

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