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Mol Cell Proteomics. 2018 Apr;17(4):764-775. doi: 10.1074/mcp.RA117.000261. Epub 2018 Jan 19.

Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag).

Author information

1
From the ‡Departments of Chemistry, cwoo@chemistry.harvard.edu.
2
§Microbiology & Immunology, and.
3
‖Interdepartmental Program in Immunology.
4
§§KAYAK, Cambridge, Massachusetts 02142.
5
‡‡Howard Hughes Medical Institute; Stanford University, Stanford, California 94305.
6
From the ‡Departments of Chemistry.
7
¶Radiology.
8
**Canary Center for Cancer Early Detection.

Abstract

Post-translational modifications (PTMs) on proteins often function to regulate signaling cascades, with the activation of T cells during an adaptive immune response being a classic example. Mounting evidence indicates that the modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), the only mammalian glycan found on nuclear and cytoplasmic proteins, helps regulate T cell activation. Yet, a mechanistic understanding of how O-GlcNAc functions in T cell activation remains elusive, partly because of the difficulties in mapping and quantifying O-GlcNAc sites. Thus, to advance insight into the role of O-GlcNAc in T cell activation, we performed glycosite mapping studies via direct glycopeptide measurement on resting and activated primary human T cells with a technique termed Isotope Targeted Glycoproteomics. This approach led to the identification of 2219 intact O-linked glycopeptides across 1045 glycoproteins. A significant proportion (>45%) of the identified O-GlcNAc sites lie near or coincide with a known phosphorylation site, supporting the potential for PTM crosstalk. Consistent with other studies, we find that O-GlcNAc sites in T cells lack a strict consensus sequence. To validate our results, we employed gel shift assays based on conjugating mass tags to O-GlcNAc groups. Notably, we observed that the transcription factors c-JUN and JUNB show higher levels of O-GlcNAc glycosylation and higher levels of expression in activated T cells. Overall, our findings provide a quantitative characterization of O-GlcNAc glycoproteins and their corresponding modification sites in primary human T cells, which will facilitate mechanistic studies into the function of O-GlcNAc in T cell activation.

PMID:
29351928
PMCID:
PMC5880114
DOI:
10.1074/mcp.RA117.000261
[Indexed for MEDLINE]
Free PMC Article

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