Format

Send to

Choose Destination
Plant Physiol Biochem. 2018 Mar;124:100-111. doi: 10.1016/j.plaphy.2018.01.008. Epub 2018 Jan 11.

Overexpression of a wheat (Triticum aestivum L.) bZIP transcription factor gene, TabZIP6, decreased the freezing tolerance of transgenic Arabidopsis seedlings by down-regulating the expression of CBFs.

Author information

1
College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang, Hebei 050024, China. Electronic address: caiwangting@jaborehoo.com.
2
College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang, Hebei 050024, China. Electronic address: martin@jdcsh.com.
3
College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang, Hebei 050024, China. Electronic address: wangweiwei92@outlook.com.
4
College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang, Hebei 050024, China. Electronic address: guoguangyan1@sina.com.
5
College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang, Hebei 050024, China. Electronic address: liuwei6382@sina.com.
6
College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang, Hebei 050024, China. Electronic address: beicaili@sina.com.

Abstract

The basic leucine zipper (bZIP) proteins play important roles against abiotic stress in plants, including cold stress. However, most bZIPs involved in plant freezing tolerance are positive regulators. Only a few bZIPs function negatively in cold stress response. In this study, TabZIP6, a Group C bZIP transcription factor gene from common wheat (Triticum aestivum L.), was cloned and characterized. The transcript of TabZIP6 was strongly induced by cold treatment (4 °C). TabZIP6 is a nuclear-localized protein with transcriptional activation activity. Arabidopsis plants overexpressing TabZIP6 showed decreased tolerance to freezing stress. Microarray as well as quantitative real-time PCR (qRT-PCR) analysis showed that CBFs and some key COR genes, including COR47 and COR15B, were down-regulated by cold treatment in TabZIP6-overexpressing Arabidopsis lines. TabZIP6 was capable of binding to the G-box motif and the CBF1 and CBF3 promoters in yeast cells. A yeast two-hybrid assay revealed that TabZIP6, as well as the other two Group S bZIP proteins involved in cold stress tolerance in wheat, Wlip19 and TaOBF1, can form homodimers by themselves and heterodimers with each other. These results suggest that TabZIP6 may function negatively in the cold stress response by binding to the promoters of CBFs, and thereby decreasing the expression of downstream COR genes in TabZIP6-overexpressing Arabidopsis seedlings.

KEYWORDS:

CBFs; Freezing tolerance; Negative regulation; TabZIP6; Transgenic Arabidopsis

PMID:
29351891
DOI:
10.1016/j.plaphy.2018.01.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center