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Am J Physiol Heart Circ Physiol. 2018 Apr 1;314(4):H724-H732. doi: 10.1152/ajpheart.00512.2017. Epub 2018 Jan 5.

Guidelines for authors and reviewers on antibody use in physiology studies.

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Department of Physiology, Pharmacology and Medicine, Sarver Heart Center, College of Medicine, University of Arizona , Tucson, Arizona.
Mississippi Center for Heart Research, Department of Physiology and Biophysics, University of Mississippi Medical Center , Jackson, Mississippi.
Research Service, G.V. (Sonny) Montgomery Veterans Affairs Medical Center , Jackson, Mississippi.


Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at .


antibody; immunoblot analysis; immunoblotting; immunohistochemistry; rigor and reproducibility

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