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J Cell Physiol. 2018 Dec;233(12):9365-9374. doi: 10.1002/jcp.26471. Epub 2018 Jun 19.

Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell.

Author information

1
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
2
The Laboratory of Synaptic Development and Plasticity, Institute of Life Science, Nanchang University, Nanchang, China.
3
School of Life Science, Nanchang University, Nanchang, China.
4
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, China.
5
Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu, China.
6
College of Life Sciences, Xinyang Normal University, Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang, Henan, China.
7
Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
8
Animal Health Supervision in Henan Province, Zhengzhou, Henan, China.
9
Bureau of Animal Husbandry of Biyang County, Biyang, Henan, China.

Abstract

Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndrome. Previous studies suggested that DEC1 was involved in skeletal muscle development; however, its precise role in myoblast differentiation has not been determined. In the present study, we showed that DEC1 expressed ubiquitously in different bovine tissues and was down-regulated in differentiated bovine satellite cells. Expression of muscle specific transcription factors (Myf5, MyoD, MyoG, and MHC) was significantly down-regulated when DEC1 was over-expressed by both CoCl2 -simulated hypoxia and Adenovirus-mediated transduction in bovine satellite cells. Consistent with that, promoter analyses via luciferase reporter assay also revealed that overexpression of bovine DEC1 could inhibit MyoG promoter activity. In conclusion, overexpression of DEC1 blocked myogenesis by inhibiting MyoG promoter activity in bovine. Our results provided a new mechanism for the muscle growth, which would contribute to increase cattle meat productivity.

KEYWORDS:

DEC1; MyoG; cattle; satellite cell; skeletal muscle

PMID:
29350420
DOI:
10.1002/jcp.26471

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