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Biomed Res Int. 2017;2017:1492327. doi: 10.1155/2017/1492327. Epub 2017 Nov 16.

Abdominal Manual Therapy Repairs Interstitial Cells of Cajal and Increases Colonic c-Kit Expression When Treating Bowel Dysfunction after Spinal Cord Injury.

Author information

1
Rehabilitation Therapy Department, Hainan Provincial Nongken General Hospital, Haikou, Hainan 570100, China.
2
The Second Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, China.
3
Rehabilitation Medicine Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, China.
4
Rehabilitation Department, Yixing Jiuru Rehabilitation Hospital, Yixing, Jiangsu 214200, China.
5
Department of Orthopedics & Traumatology, Jiangyin Orthopedics Hospital of Traditional Chinese Medicine, Jiangyin, Jiangsu 214400, China.
6
Rehabilitation Department, Wuxi Hand Surgery Hospital, Wuxi, Jiangsu 214000, China.

Abstract

Background:

This study aimed to evaluate the therapeutic effects of abdominal manual therapy (AMT) on bowel dysfunction after spinal cord injury (SCI), investigating interstitial cells of Cajal (ICCs) and related c-kit expression.

Methods:

Model rats were divided as SCI and SCI with drug treatment (intragastric mosapride), low-intensity (SCI + LMT; 50 g, 50 times/min), and high-intensity AMT (SCI + HMT; 100 g, 150 times/min). After 14 days of treatment, weight, improved Basso-Beattie-Bresnahan (BBB) locomotor score, and intestinal movement were evaluated. Morphological structure of spinal cord and colon tissues were examined. Immunostaining, RT-PCR, and western blot were used to assess c-kit expression.

Results:

In SCI rats, AMT could not restore BBB, but it significantly increased weight, shortened time to defecation, increased feces amounts, and improved fecal pellet traits and colon histology. AMT improved the number, distribution, and ultrastructure of colonic ICCs, increasing colonic c-kit mRNA and protein levels. Compared with the SCI + Drug and SCI + LMT groups, the SCI + HMT group showed better therapeutic effect in improving intestinal transmission function and promoting c-kit expression.

Conclusions:

AMT is an effective therapy for recovery of intestinal transmission function. It could repair ICCs and increase c-kit expression in colon tissues after SCI, in a frequency-dependent and pressure-dependent manner.

PMID:
29349063
PMCID:
PMC5733934
DOI:
10.1155/2017/1492327
[Indexed for MEDLINE]
Free PMC Article

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