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Sci Rep. 2018 Jan 18;8(1):1152. doi: 10.1038/s41598-018-19582-w.

Viral highway to nucleus exposed by image correlation analyses.

Author information

1
Department of Biological and Environmental Science and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland.
2
Laboratory for Fluorescence Dynamics, University of California, Irvine, California, USA.
3
Department of Biomedical Engineering, University of California, Irvine, California, USA.
4
Department of Physics, and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, FI-40500, Finland.
5
Baker Institute for Animal Health, College of Veterinary Medicine, University of Cornell, Ithaca, USA.
6
Institute of Physiology II, University of Münster, Münster, Germany.
7
University of Bordeaux, Microbiologie Fondamentale et Pathogénicité, Bordeaux, France.
8
Department of Biological and Environmental Science and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland. maija.vihinen-ranta@jyu.fi.

Abstract

Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm. The unique combination of image analyses allowed us to follow the fate of intracellular single virus particles and their interactions with importin β revealing previously unknown dynamics of the entry pathway.

PMID:
29348472
PMCID:
PMC5773500
DOI:
10.1038/s41598-018-19582-w
[Indexed for MEDLINE]
Free PMC Article

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