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Hum Mol Genet. 2018 Mar 15;27(6):1039-1054. doi: 10.1093/hmg/ddy023.

Analysis of experience-regulated transcriptome and imprintome during critical periods of mouse visual system development reveals spatiotemporal dynamics.

Author information

1
Graduate Institute of Brain and Mind Sciences, College of Medicine, National Taiwan University, Taipei 10051, Taiwan.
2
Department of Pediatrics, Yong-He Cardinal Tien Hospital, Taipei 234, Taiwan.
3
Department of Life Science, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
4
Department of Pharmacology, School of Medical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
5
Institute of Biotechnology, College of Bio-Resources & Agriculture, National Taiwan University, Taipei 106, Taiwan.
6
Department of Ophthalmology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan.
7
Neurodevelopment Club in Taiwan, Taipei 10051, Taiwan.
8
Department of Psychiatry, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei 10051, Taiwan.

Abstract

Visual system development is light-experience dependent, which strongly implicates epigenetic mechanisms in light-regulated maturation. Among many epigenetic processes, genomic imprinting is an epigenetic mechanism through which monoallelic gene expression occurs in a parent-of-origin-specific manner. It is unknown if genomic imprinting contributes to visual system development. We profiled the transcriptome and imprintome during critical periods of mouse visual system development under normal- and dark-rearing conditions using B6/CAST F1 hybrid mice. We identified experience-regulated, isoform-specific and brain-region-specific imprinted genes. We also found imprinted microRNAs were predominantly clustered into the Dlk1-Dio3 imprinted locus with light experience affecting some imprinted miRNA expression. Our findings provide the first comprehensive analysis of light-experience regulation of the transcriptome and imprintome during critical periods of visual system development. Our results may contribute to therapeutic strategies for visual impairments and circadian rhythm disorders resulting from a dysfunctional imprintome.

PMID:
29346572
DOI:
10.1093/hmg/ddy023
[Indexed for MEDLINE]

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