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ACS Appl Mater Interfaces. 2018 Feb 7;10(5):4349-4358. doi: 10.1021/acsami.7b17871. Epub 2018 Jan 22.

Engineered Phage Matrix Stiffness-Modulating Osteogenic Differentiation.

Author information

1
Bioengineering, University of California, Berkeley, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
2
Ministry of Food and Drug Safety, Center for Test and Analysis , Busan 48562, Republic of Korea.
3
BIO-IT Foundry Technology Institute, Pusan National University , Busan 46241, Republic of Korea.
4
Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital , Yangsan 50612, Republic of Korea.
5
Control and Instrumentation Engineering, Korea Maritime and Ocean University , Busan 49112, Republic of Korea.
6
Genetic Engineering, Sungkyunkwan University , Suwon 16419, Republic of Korea.
7
Mechanical Engineering, Korea University , Seoul 02841, Republic of Korea.

Abstract

Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.

KEYWORDS:

differentiation; matrix; osteogenic; phage; stiffness

PMID:
29345898
DOI:
10.1021/acsami.7b17871
[Indexed for MEDLINE]

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