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Int J Mol Sci. 2018 Jan 17;19(1). pii: E280. doi: 10.3390/ijms19010280.

Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion.

Author information

1
College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea. rose_0013@naver.com.
2
College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea. zhangxiaowei9304@gmail.com.
3
College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea. vq1004pv@naver.com.
4
College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea. kkg0118@gmail.com.
5
Laboratory of Cardiovascular Regeneration, Division of Cardiology, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul 02841, Korea. tempast606@naver.com.
6
Laboratory of Cardiovascular Regeneration, Division of Cardiology, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul 02841, Korea. whitesh@catholic.ac.kr.
7
Department of Food Science and Technology, Keimyung University, Daegu 42601, Korea. bomnae1110@gmail.com.
8
Department of Food Science and Technology, Keimyung University, Daegu 42601, Korea. tnwjd0799@naver.com.
9
Department of Food Science and Technology, Keimyung University, Daegu 42601, Korea. jtkim92@kmu.ac.kr.
10
KU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea. hongkh27@gmail.com.
11
KU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 05029, Korea. hojaebae@konkuk.ac.kr.

Abstract

To overcome the drawbacks of conventional drug delivery system, nanoemulsion have been developed as an advanced form for improving the delivery of active ingredients. However, safety evaluation is crucial during the development stage before the commercialization. Therefore, the aim of this study was to evaluate the cytotoxicity of two types of newly developed nanoemulsions. Turmeric extract-loaded nanoemulsion powder-10.6 (TE-NEP-10.6, high content of artificial surfactant Tween 80), which forms the optimal nanoemulsion, and the TE-NEP-8.6 made by increasing the content of natural emulsifier (lecithin) to reduce the potential toxicity of nanoemulsion were cultured with various cells (NIH3T3, H9C2, HepG2, hCPC, and hEPC) and the changes of each cell were observed followed by nanoemulsion treatment. As a result, the two nanoemulsions (TE-NEP-10.6 and TE-NEP-8.6) did not show significant difference in cell viability. In the case of cell line (NIH3T3, H9C2, and HepG2), toxicity was not observed at an experimental concentration of less than 1 mg/mL, however, the cell survival rate decreased in a concentration dependent manner in the case of primary cultured cells. These results from our study can be used as a basic data to confirm the cell type dependent toxicity of nanoemulsion.

KEYWORDS:

curcumin; cytotoxicity; nanoemulsion; turmeric

PMID:
29342111
PMCID:
PMC5796226
DOI:
10.3390/ijms19010280
[Indexed for MEDLINE]
Free PMC Article

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