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Chembiochem. 2018 Jun 18;19(12):1232-1238. doi: 10.1002/cbic.201700630. Epub 2018 Feb 27.

Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging.

Author information

1
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, 24 rue Lhomond, 75005, Paris, France.
2
IBENS, CNRS-UMR8197, INSERM-U1024, PSL Research University, 46 rue d'Ulm, 75005, Paris, France.
3
Department of Chemical and Systems Biology, Stanford University, Stanford, CA, 94305, USA.
4
PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University, 75005, Paris, France.
5
Sorbonne Universités, UPMC Univ Paris 06, École Normale Supérieure, CNRS, PASTEUR, 75005, Paris, France.
6
Sorbonne Universités, UPMC Univ Paris 06, 4 place Jussieu, 75005, Paris, France.
7
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA, 90024, USA.
8
Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS UMR 7241, INSERM U1050, 11 place Marcellin Berthelot, 75005, Paris, France.
9
Department of Biology, Ecole Normale Supérieure, PSL Research University, 46 rue d'Ulm, 75005, Paris, France.
10
Department of Life Sciences, Paris-Diderot University, Sorbonne-Paris-Cité, 5 rue Thomas Mann, 75013, Paris, France.

Abstract

The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.

KEYWORDS:

caged compounds; cyclofen-OH; gene expression; optogenetics; photolysis

PMID:
29341391
DOI:
10.1002/cbic.201700630

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