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Eur J Pharm Sci. 2018 Apr 30;116:61-69. doi: 10.1016/j.ejps.2018.01.025. Epub 2018 Jan 12.

A novel biphenolic ligand for selective Mg2+ and Zn2+ ions sensing followed by colorimetric, spectroscopic and cell imaging methods.

Author information

1
Department of Chemistry, National Institute of Technology, Tiruchirappalli 620015, Tamilnadu, India. Electronic address: puma@nitt.edu.
2
Department of Chemistry, Selvamm College of Technology, Namakkal 637003, Tamilnadu, India.
3
Department of Pharmaceutical Technology, Centre for Excellence in Nanobio Translational Research (CENTRE), Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620024, Tamil Nadu, India.

Abstract

The (E)-2-((2-hydrohy-5-methylphenylimino) methyl) phenol ligand was synthesized. The receptor was characterized by IR, 1H and 13C NMR and CHN analysis. The ligand exhibits colorimetric and fluorometric sensing of Zn2+ and Mg2+ ions in semi-aqueous medium (DMSO-H2O). The receptor was tested with series of transition metal ions (Cr2+, Fe2+, Ni2+, Co2+, Cu2+, Zn2+) and heavy metal ions (Sn2+, Pd2+, Ce2+, Hg2+, Cd2+) and the essential human body elements like Ca2+, Mg2+, Na+ and K+ ions. The naked eye colorimetric sensing was absorbed only for Zn2+ and Mg2+. Both ions (ZnCl2 and MgCl2 in H2O), when added to the colorless solutions of the receptor of about 1 equivalence in incremental additions turn the solution into bright turmeric yellow. All other ions remain inactive, in colorimetric sensing. Further the Zn2+ and Mg2+ ions were probed by absorption and emission spectroscopy through incremental addition of respective metal ions. The in-situ deprotonation of the ligand on both Mg2+ and Zn2+ ions binding was confirmed by 1H NMR titration studies. The imino nitrogen of the receptor is not coordinated to the metal ions. The Job's plot studies reveal the 1:2 binding ratio of metal ions to the receptor. The high fold fluorescence output on metal ions binding was positively used to sense the Zn2+ and Mg2+ ions, separately and together in HeLa cancer cells through cell imaging.

KEYWORDS:

Colorimetric; Fluorescence microscopy; Live cell imaging; MgCl(2); ZnCl(2)

PMID:
29339227
DOI:
10.1016/j.ejps.2018.01.025
[Indexed for MEDLINE]

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