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BMC Genomics. 2018 Jan 16;19(1):51. doi: 10.1186/s12864-017-4402-2.

Soybean MADS-box gene GmAGL1 promotes flowering via the photoperiod pathway.

Author information

1
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
2
College of Agro-grass-land Science, Nanjing Agricultural University, Nanjing, Jiangsu, China.
3
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. fhuang@njau.edu.cn.
4
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. dyyu@njau.edu.cn.

Abstract

BACKGROUND:

The MADS-box transcription factors are an ancient family of genes that regulate numerous physiological and biochemical processes in plants and facilitate the development of floral organs. However, the functions of most of these transcription factors in soybean remain unknown.

RESULTS:

In this work, a MADS-box gene, GmAGL1, was overexpressed in soybean. Phenotypic analysis showed that GmAGL1 overexpression not only resulted in early maturation but also promoted flowering and affected petal development. Furthermore, the GmAGL1 was much more effective at promoting flowering under long-day conditions than under short-day conditions. Transcriptome sequencing analysis showed that before flowering, the photoperiod pathway photoreceptor CRY2 and several circadian rhythm genes, such as SPA1, were significantly down-regulated, while some other flowering-promoting circadian genes, such as GI and LHY, and downstream genes related to flower development, such as FT, LEAFY, SEP1, SEP3, FUL, and AP1, were up-regulated compared with the control. Other genes related to the flowering pathway were not noticeably affected.

CONCLUSIONS:

The findings reported herein indicate that GmAGL1 may promote flowering mainly through the photoperiod pathway. Interestingly, while overexpression of GmAGL1 promoted plant maturity, no reduction in seed production or oil and protein contents was observed.

KEYWORDS:

Flowering; MADS-box gene; Photoperiod pathway; Soybean; Transgenic

PMID:
29338682
PMCID:
PMC5769455
DOI:
10.1186/s12864-017-4402-2
[Indexed for MEDLINE]
Free PMC Article

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