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Vector Borne Zoonotic Dis. 2018 Jan;18(1):21-30. doi: 10.1089/vbz.2017.2192.

Studies of Transgenic Mosquitoes in Disease-Endemic Countries: Preparation of Containment Facilities.

Author information

1
1 Centre for Environmental Policy, Imperial College London , Ascot, United Kingdom .
2
2 International Centre of Insect Physiology and Ecology , Mbita Point, Kenya .
3
3 Department of Biological Sciences, Mount Kenya University , Thika, Kenya .
4
4 Institut de Recherche en Sciences de la Santé , (IRSS)/Centre Muraz, Bobo Dioulasso, Burkina Faso .
5
5 Malaria Research and Training Center, Université des Sciences, des Techniques et des Technologies de Bamako (MRTC/USTTB) , Bamako, Mali .
6
6 Uganda Virus Research Institute (UVRI) , Entebbe, Uganda .
7
7 Department of Life Sciences, Imperial College London , Ascot, United Kingdom .
8
8 Entomology Branch, Centers for Disease Control and Prevention (CDC) , Atlanta, Georgia .
9
9 Donald Danforth Plant Science Center , St. Louis, Missouri.

Abstract

Novel approaches to area-wide control of vector species offer promise as additional tools in the fight against vectored diseases. Evaluation of transgenic insect strains aimed at field population control in disease-endemic countries may involve international partnerships and should be done in a stepwise approach, starting with studies in containment facilities. The preparations of both new-build and renovated facilities are described, including working with local and national regulations regarding land use, construction, and biosafety requirements, as well as international guidance to fill any gaps in regulation. The examples given are for containment categorization at Arthropod Containment Level 2 for initial facility design, classification of wastes, and precautions during shipping. Specific lessons were derived from preparations to evaluate transgenic (non-gene drive) mosquitoes in West and East African countries. Documented procedures and the use of a non-transgenic training strain for trial shipments and culturing were used to develop competence and confidence among the African facility staff, and along the chain of custody for transport. This practical description is offered to support other research consortia or institutions preparing containment facilities and operating procedures in conditions where research on transgenic insects is at an early stage.

KEYWORDS:

biosafety; compliance; containment; insectary; mosquitoes; transgenic

PMID:
29337662
PMCID:
PMC5770122
DOI:
10.1089/vbz.2017.2192
[Indexed for MEDLINE]
Free PMC Article

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