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Chem Biol Interact. 2018 Feb 25;282:85-92. doi: 10.1016/j.cbi.2018.01.013. Epub 2018 Jan 12.

The in vitro radiosensitizer potential of resveratrol on MCF-7 breast cancer cells.

Author information

1
Laboratory of Experimental Physiology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
2
Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
3
University Hospital of Santa Maria, Federal University of Santa Maria, Santa Maria, RS, Brazil.
4
Center of Health Sciences, Lutheran University of Brazil, Canoas, RS, Brazil.
5
Laboratory of Experimental Physiology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: lgfbauer@smail.ufsm.br.

Abstract

Radiation therapy is commonly applied in breast cancer (BC) patients. However, radioresistance and side effects are limiting factors of this practice. Therefore, studying substances that can enhance the radiation effect and, at the same time, protect normal cells is very relevant. Thus, the aim of this work was to assess the radiosensitizer effect of resveratrol (RV) on BC cells (MCF-7). A high cytotoxic and antiproliferative effect was observed in the treatment with 10 μM of RV + 3 Gy ionizing radiation (IR). Our results indicate that, 24 h after the exposition of cell cultures to RV + IR, an induction of necrosis/senescence has occurred. Furthermore, was observed the activation of extrinsic apoptosis pathway through a decrease of the Bax/Bcl-2 ratio and a high activity of caspase 8. Moreover, our data show that this treatment affected the oxidative cell metabolism, increasing oxidative protein, lipid and membrane damage and also acted to decrease the antioxidant enzymes activity. The antiproliferative effect on 72 h cultures may be associated with a high expression of p53 and an interruption of cell cycle in the S phase. Therefore, our results suggest that RV is a potential radiosensitizer of MCF-7 BC cells.

KEYWORDS:

Antioxidant; Breast cancer; Polyphenols; Radiomodifier; Radiotherapy

PMID:
29336987
DOI:
10.1016/j.cbi.2018.01.013
[Indexed for MEDLINE]
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