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Sci Rep. 2018 Jan 15;8(1):723. doi: 10.1038/s41598-018-19248-7.

Salient type 1 interleukin 1 receptor expression in peripheral non-immune cells.

Author information

1
Department of Oncolgy, Tongji Hospital, Huazhong University of Science and Technology Tongji Medical College, Wuhan, Hubei, 430030, P. R. China.
2
Institute for Behavioral Medicine Research, College of Medicine, The Ohio State University, Columbus, OH, 43210, USA.
3
West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.
4
School of Health and Rehabilitation Sciences, Division of Medical Laboratory Science, College of Medicine, Wexner Medical Center, The Ohio State University, Columbus, OH, 43210, USA.
5
Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, 43210, USA.
6
Department of Neuroscience, The Ohio State University, Columbus, OH, 43210, USA.
7
Center for Brain and Spinal Cord Repair, The Ohio State University, Columbus, OH, 43210, USA.
8
Institute for Behavioral Medicine Research, College of Medicine, The Ohio State University, Columbus, OH, 43210, USA. liu.1933@osu.edu.
9
Division of Biosciences, College of Dentistry, The Ohio State University, Columbus, OH, 43210, USA. liu.1933@osu.edu.
10
Institute for Behavioral Medicine Research, 460 Medical Center Drive, Columbus, OH, 43210, USA. liu.1933@osu.edu.
11
Institute for Behavioral Medicine Research, College of Medicine, The Ohio State University, Columbus, OH, 43210, USA. quan.14@osu.edu.
12
Division of Biosciences, College of Dentistry, The Ohio State University, Columbus, OH, 43210, USA. quan.14@osu.edu.
13
Institute for Behavioral Medicine Research, 460 Medical Center Drive, Columbus, OH, 43210, USA. quan.14@osu.edu.

Abstract

Interleukin 1 is a pleiotropic cytokine that mediates diverse functions through its receptor, type I interleukin 1 receptor (IL-1R1). Most previous studies have focused on the expression and function of IL-1R1 in immune cells. Here we performed a comprehensive mapping of IL-1R1 distribution in multiple peripheral tissues using our IL-1R1 reporter (IL-1R1GR/GR) mice. This method yielded the highest sensitivity of in situ detection of IL-1R1 mRNA and protein. Besides validating previously reported IL-1R1 expression in the endocrine tissues including pituitary and pancreas, our results refuted previously reported exclusive IL-1R1 expression in neurons of the spinal cord dorsal horn and dorsal root ganglia (DRG). Instead, IL-1R1 expression was detected in endothelial cells within DRG, spinal cord, pancreas, colon, muscles and many immune organs. In addition, gp38+ fibroblastic reticular cells (FRCs), rather than tissue macrophages or other immune cells, were found to express high levels of IL-1R1 in colon and many immune organs. A functional test of spleen FRCs showed that they responded rapidly to systemic IL-1β stimulation in vivo. Taken together, this study provides a rigorous re-examination of IL-1R1 expression in peripheral tissues and reveals tissue FRCs as a previously unappreciated novel high IL-1R1-expressing cell type in peripheral IL-1 signaling.

PMID:
29335509
PMCID:
PMC5768710
DOI:
10.1038/s41598-018-19248-7
[Indexed for MEDLINE]
Free PMC Article

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