Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease

Ekaterina Savchenko; Ana Marote; Kaspar Russ; Anna Collin; Stefano Goldwurm; Laurent Roybon; Yuriy Pomeshchik

doi:10.1016/j.scr.2018.01.002

Generation of a human induced pluripotent stem cell line (CSC-42) from a patient with sporadic form of Parkinson's disease

Stem Cell Res. 2018 Mar:27:78-81. doi: 10.1016/j.scr.2018.01.002. Epub 2018 Jan 4.

Authors

Ekaterina Savchenko¹, Ana Marote², Kaspar Russ¹, Anna Collin³, Stefano Goldwurm⁴, Laurent Roybon⁵, Yuriy Pomeshchik¹

Affiliations

¹ Stem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, Lund, Sweden; Strategic Research Area MultiPark, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden.
² Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga, Guimarães, Portugal.
³ Department of Clinical Genetics and Pathology, Office for Medical Services, Division of Laboratory Medicine, Lund, Sweden.
⁴ Parkinson Institute, ASST PINI-CTO, Milan, Italy.
⁵ Stem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, Lund, Sweden; Strategic Research Area MultiPark, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden. Electronic address: laurent.roybon@med.lu.se.

PMID: 29334629
DOI: 10.1016/j.scr.2018.01.002

Abstract

Skin fibroblasts were collected from a 44-year-old patient with sporadic case of Parkinson's disease (PD). The non-integrating Sendai virus vector encoding OCT3/4, SOX2, c-MYC and KLF4 was used to reprogram fibroblasts into induced pluripotent stem cells (iPSCs). Generated iPSCs had normal karyotypes, expressed common stem cell markers, and were capable of differentiating into all three germ layers. Generated line could be used for PD modeling to understand the mechanisms that influence the disorder.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation / genetics
Cell Differentiation / physiology
Cells, Cultured
Female
Fibroblasts / cytology
Fibroblasts / metabolism
Germ Layers / cytology
Germ Layers / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism*
Karyotype
Kruppel-Like Factor 4
Parkinson Disease / metabolism*