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Chem Commun (Camb). 2018 Jan 31;54(10):1189-1192. doi: 10.1039/c7cc08880a.

SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells.

Author information

1
Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China. xuyi70@163.com.

Abstract

A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient and shows little attenuation of viral infectivity. Therefore, it is a biologically compatible technique for tracking viral infection.

PMID:
29334085
DOI:
10.1039/c7cc08880a
[Indexed for MEDLINE]

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