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Virology. 2018 Mar;516:76-85. doi: 10.1016/j.virol.2018.01.005. Epub 2018 Jan 11.

Development of a novel equine influenza virus live-attenuated vaccine.

Author information

1
Department of Microbiology and Immunology, University of Rochester, Rochester, NY, United States.
2
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY, United States.
3
MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
4
Department of Microbiology and Immunology, University of Rochester, Rochester, NY, United States. Electronic address: luis_martinez@urmc.rochester.edu.

Abstract

H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a temperature sensitive (ts) H3N8 EIV live-attenuated influenza vaccine (LAIV) using reverse-genetics approaches. Our EIV LAIV was attenuated (att) in vivo and able to induce, upon a single intranasal administration, protection against H3N8 EIV wild-type (WT) challenge in both a mouse model and the natural host, the horse. Notably, since our EIV LAIV was generated using reverse genetics, the vaccine can be easily updated against drifting or emerging strains of EIV using the safety backbone of our EIV LAIV as master donor virus (MDV). These results demonstrate the feasibility of implementing a novel EIV LAIV approach for the prevention and control of currently circulating H3N8 EIVs in horse populations.

KEYWORDS:

Attenuated (att); Equine influenza virus (EIV); Influenza A virus (IAV); Live-attenuated influenza vaccine (LAIV); Master donor virus (MDV); Reverse genetics techniques; Temperature sensitive (ts)

PMID:
29331866
PMCID:
PMC5840510
DOI:
10.1016/j.virol.2018.01.005
[Indexed for MEDLINE]
Free PMC Article

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