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Biosens Bioelectron. 2018 May 1;104:138-144. doi: 10.1016/j.bios.2018.01.008. Epub 2018 Jan 6.

Water-soluble mercury ion sensing based on the thymine-Hg2+-thymine base pair using retroreflective Janus particle as an optical signaling probe.

Author information

1
Department of Molecular Science and Technology, Ajou University, Suwon 443749, South Korea.
2
Department of Molecular Science and Technology, Ajou University, Suwon 443749, South Korea. Electronic address: hcyoon@ajou.ac.kr.

Abstract

Herein, we report an optical sensing platform for mercury ions (Hg2+) in water based on the integration of Hg2+-mediated thymine-thymine (T-T) stabilization, a biotinylated stem-loop DNA probe, and a streptavidin-modified retroreflective Janus particle (SA-RJP). Two oligonucleotide probes, including a stem-loop DNA probe and an assistant DNA probe, were utilized. In the absence of Hg2+, the assistant DNA probe does not hybridize with the stem-loop probe due to their T-T mismatch, so the surface-immobilized stem-loop DNA probe remains a closed hairpin structure. In the presence of Hg2+, the DNA forms a double-stranded structure with the loop region via Hg2+-mediated T-T stabilization. This DNA hybridization induces stretching of the stem-loop DNA probe, exposing biotin. To translate these Hg2+-mediated structural changes in DNA probe into measurable signal, SA-RJP, an optical signaling label, is applied to recognize the exposed biotin. The number of biospecifically bound SA-RJPs is proportional to the concentration of Hg2+, so that the concentration of Hg2+ can be quantitatively analyzed by counting the number of RJPs. Using the system, a highly selective and sensitive measurement of Hg2+ was accomplished with a limit of detection of 0.027nM. Considering the simplified optical instrumentation required for retroreflection-based RJP counting, RJP-assisted Hg2+ measurement can be accomplished in a much easier and inexpensive manner. Moreover, the detection of Hg2+ in real drinking water samples including tap and commercial bottled water was successfully carried out.

KEYWORDS:

Hg(2+)-mediated thymine- thymine base pairing; Mercury ion sensing; Optical biosensor; Retroreflective Janus particles; Stem-loop DNA probe

PMID:
29331427
DOI:
10.1016/j.bios.2018.01.008
[Indexed for MEDLINE]

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